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KMID : 0061119830110000140
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Bulletin of the Clinical Research Institute
1983 Volume.11 No. 0 p.140 ~ p.140
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In vivo Expansion of Transferred Contact Sensitivity by Allogeneic Spleen Cells in guinea Pigs
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Choi Guk-Joo
Kim Chung-Won
Houh Won
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Abstract
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This animal experiment was designed not only to transfer 2,4-dinitrochlorobenzene (DNCB) contact sensitivity by spleen cells but also to achieve in vivo expansion of transferred contact sensitivity in guinea pigs.
A total of 32 guinea ´pigs were randomly divided into 8 experimental groups of transferred DNCB contact sensitivity; donor-recipient of the sensitization groups, donor-recipient of cyclophosphamide pretreated groups and donor-recipient of the boosted sensitization groups.
The results obtained were as follows;
1. The transferred contact sensitivity was reduced` in the recipient of the sensitization group to 43.05% compared with 70.83% of the positive control, however, the transferred contact sensitivity was effectively achieved as it compared with the negative control group (23.81%).
2. In vivo expansion of transferred contact sensitivity was achieved by means of sensitizing the donors by 2 different methods cyclophosphamide 100 ㎎/㎏ injection to the cyclophosphamide pretreated group and the repeated sensitization for the boosted sensitization group. In vivo expansion of transferred DNCB contact sensitivity was 63.89% in the cyclophosphamide pretreated and 66.66% in the boosted sensitization group.
3. As low doses as 100 ㎎/㎏ of cyclophosphamide intraperitoneal injection was effective for the expansion of transferred contact sensitivity by allogeneic spleen cells in guinea pigs.
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